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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AX685147.1
Database: emblrelpat Go to original record
Description: Sequence 8 from Patent WO0236831.
Taxon: synthetic construct
Original size: 474 bp
Patents: WO0236831 (lens, espacenet)

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Method: QL-EVE-BN-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of oilseed rape event GT73 (Pan et al., 2007).
Target:MON-00073-7

View other 5 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 118 321 + 204 bp
Amplicon: 204 bp 
1     aataacgctg cggacatcta catttttgaa ttgaaaaaaa attggtaatt
51    actctttctt tttctccata ttgaccatca tactcattgc tgatccatgt
101   agatttcccg gacatgaaga tcatcctcct tcctttcctt gcctttcctt
151   ccttttcttg ccttcgtata agcttgtgtc aattgttgac agagaatctt
201   gctg

View other 5 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 9.72e-09
primerB 100.0 22 bp 0 0 204 183 8.59e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.