QL-CON-00-008
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event RT73 (GT73) | MON-00073-7 (Oilseed Rape)
GMO event NK603 | MON-00603-6 (Maize) |
| Genetic target: | CP4-epsps / CTP2 |
| Description: | Qualitative PCR method for detection of the junction between the CTP2 and the CP4-epsps gene (Grohmann et al., 2009) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GGGATGACGTTAATTGGCTCTGNNNNNNNNNNNNNNNNGGTCATGTCTTCTGTTTCCACGGCGTGNNNNCTTCACGGTGC AAGCAGCC |
| Amplicon size: | 88 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | GT73-TmF / | GGGATGACGTTAATTGGCTCTG | 22 bp | CTP2 |
| Primer reverse | GT73-TmR / | GGCTGCTTGCACCGTGAAG | 19 bp | CP4-epsps |
| Probe | GT73-TmP / | FAM-CACGCCGTGGAAACAGAAGACATGACC-TAMRA | 27 bp |