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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

GM pollen DNA in honey

Verification Report on the extraction and analysis of GM pollen DNA in honey

Following the judgment of 06 September 2011 on GM honey by the European Court of Justice (legal case C-442-09), the European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF) established by Regulation (EC) No 1829/2003, performed an in-house study to test the extraction and PCR analysis of genomic DNA from genetically modified pollen in honey.

The present report documents an extraction method for isolation and analysis of pollen DNA present in honey, including the isolation and analysis of isolated genomic pollen DNA using real-time PCR on commercial honey samples and honey samples spiked with various levels of GM MON 810 pollen.

Documents

Verification Report on the extraction and analysis of GM pollen DNA in honey
Version 05/07/2012 (24 kB - PDF)
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Standard Operating Procedure DNA Extraction from honey and pollen – CTAB
Version 05/07/2012 (527 kB - PDF)
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