Match details
Record ID: FJ467933.1
Database:
emblreltgn
Go to original record
Description:
Carica papaya transgenic cultivar Rainbow beta-glucuronidase (uidA), coat protein, and neophosphotransferase genes, complete cds.
Taxon:
Carica papaya
Original size:
15595 bp
View other 63 matches for this record
Method: QL-CON-00-012
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the chimeric CMV/PRSV coat protein (ISO/FDIS 21569:2005/Amd.1:2013).
Target: CaMV P-35S-CMV/PRSV CP
View other 1 match for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
9905
10124
-
220 bp
1 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
51 tatataagga agttcatttc atttggagag gacagggtac ccctactgac
101 tatatagaga gtgtgtgtgc tgtgttttct cttttgtgtc gtagaattga
151 gtcgagtcat ggacaaatct gaatcaacca gtgctggtcg taaccatcga
201 cgtcgtcagt ccaagaatga
View other 1 match with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
3.13e-09
primerB
100.0
21 bp
0
0
220
200
3.13e-09
probe
100.0
26 bp
0
0
23
48
6.93e-12
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
