Match details

Record ID: AR184317.1
Database: emblrelpat Go to original record
Description: Sequence 9 from patent US 6342660.
Taxon: unidentified
Original size: 7378 bp
Patents: US6342660 (lens, espacenet)

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Method: QL-CON-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the intron 2 from the maize adh1 gene and the pat gene(ISO/FDIS 21569:2005).
Target:IVS2-pat

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4152 4340 + 189 bp

Amplicon: 189 bp
1     ctgggaggcc aaggtatcta atcagccatc ccatttgtga tctttgtcag
51    tagatatgat acaacaactc gcggttgact tgcgccttct tggcggctta
101   tctgtctcag gggcagactc ccgtgttccc tcggatctcg acatgtctcc
151   ggagaggaga ccagttgaga ttaggccagc tacagcagc 

View other 11 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 7.94e-10
primerB 100.0 22 bp 0 0 189 168 7.94e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.