European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record A60109.1 details
Record ID: A60109.1
Database: emblrelpat Go to original record
Description: Sequence 2 from Patent WO9706267.
Taxon: Plasmid PTS172
Original size: 6548 bp
Patents: WO9706267 (lens, espacenet)

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Method QL-ELE-00-001 details
Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target: CaMV P-35S

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 5413 5607 + 195 bp

Amplicon size
Amplicon: 195 bp
1     GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCTAT CATTCAAGAT
51    GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT
101   CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT
151   GTGACATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATC 

View other 4005 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 5413 5431 2.35e-07
primerB 100.0 20 bp 0 0 5607 5588 6.48e-08


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.