European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record A59870.1 details
Record ID: A59870.1
Database: emblrelpat Go to original record
Description: Sequence 2 from Patent WO9706269.
Taxon: unidentified
Original size: 1138 bp
Patents: WO9706269 (lens, espacenet)

View other 14 matches for this record


Method QL-ELE-00-001 details
Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target: CaMV P-35S

View other 10093 matches for this method


Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 522 717 + 196 bp

Amplicon size
Amplicon: 196 bp
1     GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCCAT CGTTGAAGAT
51    GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT
101   CGTGGAAAAA AGAAGACGTT CCAACCACGT CTTCAAAGCA AGTGGATTGA
151   TGTGATATCT CCACTGACGT AAGGGATGAC GCACAATCCC ACTATC 

View other 7 matches with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 194 212 4.43e-08
primerA 100.0 19 bp 0 0 522 540 4.43e-08
primerB 100.0 20 bp 0 0 717 698 1.21e-08


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.