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Match details


QL-ELE-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998). Targets:T-nos<--->T-nos
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Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
View other 15 matches for this record

Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 3753 3932 + 180


1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tagcgcgcaa actaggataa  
View other 27765 matches with an identical amplicon

Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 20 0 0 1 20 8.54e-09
primerB 100.000 20 0 0 180 161 8.54e-09

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link

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