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Match details


QT-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter. Targets:CaMV P-35S<--->CaMV P-35S
View other 13959 matches for this method


Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
View other 15 matches for this record

Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
1 0 2889 2970 + 82


1     ccctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 
View other 3 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 17 0 0 2 18 1.63e-07
primerB 100.000 22 0 0 82 61 3.55e-10
probe 100.000 22 0 0 21 42 3.55e-10

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link

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