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Match details

Method:

QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001). Targets:T-nos<--->T-nos
View other 14526 matches for this method


Target:

HQ233646.1
Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
Patents:
View other 15 matches for this record


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 3833 3950 + 118


Amplicon:

118bp
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc 
View other 14191 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 24 0 0 1 24 4.22e-11
primerB 100.000 24 0 0 118 95 4.22e-11

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


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