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Match details

Method:

QT-EVE-ZM-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event Bt11 (Mazzara et al., 2005). Targets:insert<--->3' junction GMO-UID:SYN-BT011-1
View other 1 match for this method


Target:

AY123624.1
Database:nt Go to original record
Description:Zea mays transgenic Pat (Pat) gene, partial cds.
Taxon:Zea mays
Original size:747 bp
Patents:
View other 6 matches for this record


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 382 451 + 70


Amplicon:

70bp
1     gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
51    ctcatggagg gattcttgga  
View other 1 match with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 20 0 0 1 20 3.42e-09
primerB 100.000 20 0 0 70 51 3.42e-09
probe 100.000 27 0 0 28 54 6.44e-13

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


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