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Match details

Method:

QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005). Targets:CaMV P-35S<--->CaMV P-35S
View other 9541 matches for this method


Target:

HQ233646.1
Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
Patents:
View other 15 matches for this record


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 2965 3043 + 79


Amplicon:

79bp
1     cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga 
View other 2987 matches with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 22 0 0 1 22 3.41e-10
primerB 100.000 22 0 0 79 58 3.41e-10
probe 100.000 26 0 0 31 56 2.52e-12

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


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