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Match details

Method:

QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001). Targets:CaMV P-35S<--->CaMV P-35S
View other 9147 matches for this method


Target:

HQ233646.1
Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
Patents:
View other 15 matches for this record


Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 2962 3084 + 123


Amplicon:

123bp
1     ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga 
View other 2961 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 21 0 0 1 21 1.84e-09
primerB 100.000 25 0 0 123 99 1.30e-11

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link


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