QT-TAX-ZM-007
|
General information
| Tested in: |
Maize
|
| Genetic target: | HMG |
| Description: | Quantitative droplet digital PCR method for detection of maize high-mobility-group gene (HMG) (Gatto et al., 2022) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Digital PCR (Simplex) |
| Amplicon sequence: |
TTGGACTAGAAATCTCGTGCTGATTAATTGTTTTACGCGTGCGTTTGTGTGGATTGTAGGACAAGGCTCCCTATGTAGC Genbank ID: AJ131373.1 |
| Amplicon size: | 79 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | ZM1-F / | TTGGACTAGAAATCTCGTGCTGA | 23 bp | HMG |
| Primer reverse | ZM1-R / | GCTACATAGGGAGCCTTGTCCT | 22 bp | HMG |
| Probe | Probe / | HEX-CAATCCACACAAACGCACGCGTA-TAMRA | 23 bp |
