QT-TAX-ZM-006
|
General information
| Tested in: |
Maize
|
| Genetic target: | zSSIIb |
| Description: | Quantitative PCR method for detection of maize starch synthase IIb gene (zSSIIb) (Shindo et al., 2002) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CTCCCAATCCTTTGACATCTGCTCCGAAGCAAAGTCAGAGCGCTGCAATGCAAAACGGAACGAGTGGGGGCAGCAGCGCG AGCACCGCCGCGCCGGTGTCCGGACCCAAAGCTGATCATCCATCAGCTCCTGTCACCAAGAGAGAAATCGA Genbank ID: AF019297 |
| Amplicon size: | 151 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | SSllb 1-5' / | CTCCCAATCCTTTGACATCTGC | 22 bp | zSSIIb |
| Primer reverse | SSIIb 1-3' / | TCGATTTCTCTCTTGGTGACAGG | 23 bp | zSSIIb |
| Probe | SSIIb-Taq / | FAM-AGCAAAGTCAGAGCGCTGCAATGCA-TAMRA | 25 bp |
