QT-TAX-ZM-004
|
General information
| Tested in: |
Maize
|
| Genetic target: | ALD1 |
| Description: | Quantitative PCR method for detection of maize aldolase 1 gene (ALD1) (EURL GMFF, 2016) |
| Comments: | A second copy of this locus is present on chromosome 8, carrying a G nucleotide in positions 31 and 64 of the amplicon sequence. Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
AGGGAGGACGCCTCCCTCCTTGAGGACATCAACAAAAGGCTTGCCATCCTTGGTCTTCTGGTACAGGGT Genbank ID: NM_001111866 |
| Amplicon size: | 69 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Aldolase primer F / | AGGGAGGACGCCTCCCT | 17 bp | ALD1 |
| Primer reverse | Aldolase primer R / | ACCCTGTACCAGAAGACCAAGG | 22 bp | ALD1 |
| Probe | Aldolase probe / | VIC-TGAGGACATCAACAAAAGGCTTGCCA-TAMRA | 26 bp |
