QT-TAX-GM-009
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Quantitative PCR method for detection of soybean lectin gene (LE1) (EURL GMFF, 2007) |
| Comments: | Nucleotide changes have been observed in EMBL record with accession number K00821 in positions 2 and 3 of the amplicon sequence. Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CWYCTTTCTCGCACCAATTGACACTAAGCCACAAACACATGCAGGTTATCTTGGTCTTTTCAACGAAAACGAGTCTGGTG ATCAAGTCGTCGCTGTTGAGTTTGA |
| Amplicon size: | 105 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | KVM164 / | CACCTTTCTCGCACCAATTGACA | 23 bp | LE1 |
| Primer reverse | KVM165 / | TCAAACTCAACAGCGACGAC | 20 bp | LE1 |
| Probe | TM021 / | FAM-CCACAAACACATGCAGGTTATCTTGG-TAMRA | 26 bp |
