Tested in:	Soybean
Genetic target:	LE1
Description:	Quantitative PCR method for detection of soybean lectin gene (LE1) (Shindo et al., 2002)
Comments:	Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties.

Specificity:	Species specific
Analysis type:	Quantitative
Assay:	Real-time PCR (Simplex)
Amplicon sequence:	GCCCTCTACTCCACCCCCATCCACATTTGGGACAAAGAAACCGGTAGCGTTGCCAGCTTCGCCGCTTCCTTCAACTTCAC CTTCTATGCCCCTGACACAAAAAGGCTTGCAGATGGGC Genbank ID: K00821
Amplicon size:	118 bp
Oligos:

Type	Name(s)	Sequence	Length	Target
Primer forward	Le1n02-5' /	GCCCTCTACTCCACCCCCA	19 bp	LE1
Primer reverse	Le1n02-3' /	GCCCATCTGCAAGCCTTTTT	20 bp	LE1
Probe	Le1-Taq /	FAM-AGCTTCGCCGCTTCCTTCAACTTCAC-TAMRA	26 bp

QT-CON-00-001