QT-TAX-GM-005
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Quantitative PCR method for detection of soybean lectin (LE1) gene (EURL GMFF, 2023) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CCAGCTTCGCCGCTTCCTTCAACTTCACCTTCTATGCCCCTGACACAAAAAGGCTTGCAGATGGGCTTGCCTTC Genbank ID: K00821 |
| Amplicon size: | 74 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | lecF / Le1 for2 | CCAGCTTCGCCGCTTCCTTC | 20 bp | LE1 |
| Primer reverse | lecR / GMO3-126Rev | GAAGGCAAGCCCATCTGCAAGCC | 23 bp | LE1 |
| Probe | lecP / Le1 Probe | FAM-CTTCACCTTCTATGCCCCTGACAC-BHQ1 | 24 bp |
