QT-TAX-GM-004
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Quantitative PCR method for detection of soybean lectin gene (LE1) (Hird et al., 2003) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Multiplex) |
| Amplicon sequence: |
TGGTCGCGCCCTCTACTCCACCCCCATCCACATTTGGGACAAAGAAACCGGTAGCGTTGCCAGCTTCGCC Genbank ID: K00821 |
| Amplicon size: | 70 bp |
| Multiplex method: |
QT-TAX-GM-004
+
QT-CON-00-002
|
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Soya lectin-F / | TGGTCGCGCCCTCTACTC | 18 bp | LE1 |
| Primer reverse | Soya lectin-R / | GGCGAAGCTGGCAACG | 16 bp | LE1 |
| Probe | Soya lectin / | VIC-CTACCGGTTTCTTTGTCCCAAATGTGGAT-TAMRA | 29 bp |