QT-TAX-GM-003
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Quantitative PCR method for detection of soybean lectin (LE1) gene (EURL GMFF, 2020) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
CTTTCTCGCACCAATTGACACTAAGCCACAAACACATGCAGGTTATCTTGGTCTTTTCAACGAAAACGAGTCTGGTGATC AAGTCGTCGCTGTTGAGTTTGA Genbank ID: K00821 |
| Amplicon size: | 102 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | KVM164 / | CTTTCTCGCACCAATTGACA | 20 bp | LE1 |
| Primer reverse | KVM165 / | TCAAACTCAACAGCGACGAC | 20 bp | LE1 |
| Probe | TM1242 / | JOE-CCACAAACACATGCAGGTTATCTTGG-BHQ1 | 26 bp | LE1 |
