QT-TAX-GM-001
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Quantitative PCR method for detection of soybean lectin gene (LE1) (Pauli et al., 2001) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
TCCACCCCCATCCACATTTGGGACAAAGAAACCGGTAGCGTTGCCAGCTTCGCCGCTTCCTTCAACTTCACCTTCTATGC C Genbank ID: K00821 |
| Amplicon size: | 81 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Lectin-F / | TCCACCCCCATCCACATTT | 19 bp | LE1 |
| Primer reverse | Lectin-R / | GGCATAGAAGGTGAAGTTGAAGGA | 24 bp | LE1 |
| Probe | Lectin-TMP / | FAM-AACCGGTAGCGTTGCCAGCTTCG-TAMRA | 23 bp |
