QT-TAX-GH-020
|
General information
| Tested in: |
Cotton
|
| Genetic target: | AdhC |
| Description: | Quantitative PCR method for detection of cotton alcohol dehydrogenase C (AdhC) gene (EURL GMFF, 2020) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CACATGACTTAGCCCATCTTTGCTTGCAGGTTTTGGTGCCACTGTGAATGTTGCTAAACCAAAAAAGGGTGGG Genbank ID: AF036569 |
| Amplicon size: | 73 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | KVM157 / | CACATGACTTAGCCCATCTTTGC | 23 bp | AdhC |
| Primer reverse | KVM158 / | CCCACCCTTTTTTGGTTTAGC | 21 bp | AdhC |
| Probe | TM1304 / | JOE-TGCAGGTTTTGGTGCCACTGTGAATG-BHQ1 | 26 bp | AdhC |
