QT-TAX-GH-017
|
General information
| Tested in: |
Cotton
|
| Genetic target: | SAH7 |
| Description: | Quantitative PCR method for detection of putative cotton SAH7 protein gene, D subgenome (EURL GMFF, 2020) |
| Comments: | The cotton endogenous gene SAH7 is present on both cotton subgenomes A and D. Primers and probe for this cotton-specific reference system match perfectly to both subgenome gene copies but amplify a 115-bp fragment from the A-subgenome and a 123-bp fragment from the D-subgenome. Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
AGTTTGTAGGTTTTGATGTTACATTGAGTTAAAGTGAATGATAGGGTGTCTAAACATAAAATAATGGGAACAACCATGAC ATGTTGAACTGGAGGTCAAAATCAGTAGGCGGTTCAAAGATGC Genbank ID: AY117068.1 |
| Amplicon size: | 123 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Sah7-uni-f1 / | AGTTTGTAGGTTTTGATGTTACATTGAG | 28 bp | SAH7 |
| Primer reverse | Sah7-uni-r1 / | GCATCTTTGAACCGCCTACTG | 21 bp | SAH7 |
| Probe | Sah7-uni-s1 / | VIC-AAACATAAAATAATGGGAACAACCATGACATGT-TAMRA | 33 bp | SAH7 |
