QT-TAX-BV-001
|
General information
| Tested in: |
Sugar Beet
|
| Genetic target: | GS2 |
| Description: | Quantitative PCR method for detection of sugar beet glutamine synthetase GS2 gene (EURL GMFF, 2024) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GACCTCCATATTACTGAAAGGAAGTCAAAAGGGATCAATTAAGTGCTCTACGAAGTTTAAAGTATGTGCCGCTCTCAAGA CTGAACATGGCACTGTGAACAGGATGGAGCAATTACTC |
| Amplicon size: | 118 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | GluA3-F / | GACCTCCATATTACTGAAAGGAAG | 24 bp | GS2 |
| Primer reverse | GluA3-R / | GAGTAATTGCTCCATCCTGTTCA | 23 bp | GS2 |
| Probe | GluD1-probe BHQ / | FAM-CTACGAAGTTTAAAGTATGTGCCGCTC-BHQ1 | 27 bp |
