QT-TAX-BN-002
|
General information
| Tested in: |
Oilseed Rape
|
| Genetic target: | BnC1 |
| Description: | Quantitative PCR method for detection of oilseed rape cruciferin storage protein (BnC1) (EURL GMFF, 2013) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GCTTCCGTGATATGCACCAGAAAGTGGAGCACATAAGGACTGGGGACACCATCGCTACACATCCCGGTGTAGCCCAAT Genbank ID: X59294 |
| Amplicon size: | 78 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | ccf R / | GCTTCCGTGATATGCACCAGAAAG | 24 bp | BnC1 |
| Primer reverse | ccf F / | ATTGGGCTACACCGGGATGTGT | 22 bp | BnC1 |
| Probe | ccf P / | VIC-CGATGGTGTCCCCAGTCCTTATGTGCTC-TAMRA | 28 bp |
