QT-EVE-ZM-040
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event DAS1131 | DAS-01131-3 (Maize)
|
| Genetic target: | 5' host genome / DAS1131 insert |
| Description: | Quantitative PCR method for detection of maize event DAS1131 (EURL GMFF, 2024) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
CTAAGAGCTAAGATTGCGCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNACATATTTTTTGAGGATAACAGCANNNNNNCA CCAAAAGTTAGGCCCGAA |
| Amplicon size: | 98 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | PHN201280 / | CTAAGAGCTAAGATTGCGCGG | 21 bp | 5'-host genome |
| Primer reverse | PHN201282 / | TTCGGGCCTAACTTTTGGTG | 20 bp | insert |
| Probe | PHN201284 / | 6-FAM-ACATATTTTTTGAGGATAACAGCA-MGB | 24 bp |
