QT-EVE-ZM-039
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event DP51291 | DP-051291-2 (Maize)
|
| Genetic target: | DP51291 insert |
| Description: | Quantitative PCR method for detection of maize event DP51291 (EURL GMFF, 2024) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CGCAATGTGTTATTAAGTTGTCTAAGCNNNNNTTTGTTTACACCACATACACGNNNNNGCATTTGGTACAGCTAACCGTG |
| Amplicon size: | 80 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | DP-051291-2 Forward Primer / | CGCAATGTGTTATTAAGTTGTCTAAGC | 27 bp | insert |
| Primer reverse | DP-051291-2 Reverse Primer / | CACGGTTAGCTGTACCAAATGC | 22 bp | 3'-host genome |
| Probe | DP-051291-2 Probe / | FAM-TTTGTTTACACCACATACACG-MGBNFQ | 21 bp |
