QT-EVE-ZM-030
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON87429 | MON-87429-9 (Maize)
|
| Genetic target: | MON87429 insert |
| Description: | Quantitative PCR method for detection of maize event MON87429 (EURL GMFF, 2021) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CGAGACAGACTCAATGTATCCGAGATACTCNNNNNNNNNNNNNNNNNNNNNNNNNGACCACTCTTGTTTGGTTTCATGTC CGGGANNNNTACATGGATCAGCAATGAGTATGATGG |
| Amplicon size: | 116 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MON 87429 primer 1 / | CGAGACAGACTCAATGTATCCGAGATACTC | 30 bp | 5'-host genome |
| Primer reverse | MON 87429 primer 2 / | CCATCATACTCATTGCTGATCCATGTA | 27 bp | insert |
| Probe | MON 87429 probe / | FAM-TCCCGGACATGAAACCAAACAAGAGTGGTC-TAMRA | 30 bp |
