QT-EVE-ZM-029
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON87419 | MON-87419-8 (Maize)
|
| Genetic target: | MON87419 insert |
| Description: | Quantitative PCR method for detection of maize event MON87419 (EURL GMFF, 2019) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CGGTCGCTGCCAGGTATTGNTGTGCGCCAGTCAGCATCATCACACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAAG CTCGCAATTGAGGTCTG |
| Amplicon size: | 97 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 87419 QF / | CGGTCGCTGCCAGGTATTG | 19 bp | insert |
| Primer reverse | 87419 QR / | CAGACCTCAATTGCGAGCTTTCT | 23 bp | insert |
| Probe | 87419 QP / | FAM-TGTGCGCCAGTCAGCATCATCACACC-TAMRA | 26 bp |
