QT-EVE-ZM-028
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MZIR098 | SYN-00098-3 (Maize)
|
| Genetic target: | MZIR098 insert |
| Description: | Quantitative PCR method for detection of maize event MZIR098 (EURL GMFF, 2018) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
ATCTCAGACACCAAACCGAGATCCAAGTGACAGCGAACGGAGCTGGTTTNNACTGGCACTAGCCTAACGGTGT |
| Amplicon size: | 73 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MZIR098 forward primer / | ATCTCAGACACCAAACCGAGATC | 23 bp | 5'-host genome |
| Primer reverse | MZIR098 reverse primer / | ACACCGTTAGGCTAGTGCCAGT | 22 bp | insert |
| Probe | MZIR098 probe / | FAM-CAAGTGACAGCGAACGGAGCTGGTTT-BHQ1 | 26 bp |
