QT-EVE-ZM-027
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MZHG0JG | SYN-000JG-2 (Maize)
|
| Genetic target: | MZHG0JG insert |
| Description: | Quantitative PCR method for detection of maize event MZHG0JG (EURL GMFF, 2018) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CAACTAGCTAGATTAATTAACGCAATCTGNNNTTAAGTTGTCTAAGCGTCAATTTGNNNNTCCCACACCTTGCAAACAAA T |
| Amplicon size: | 81 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MZHG0JG forward primer / | CAACTAGCTAGATTAATTAACGCAATCTG | 29 bp | insert |
| Primer reverse | MZHG0JG reverse primer / | ATTTGTTTGCAAGGTGTGGGA | 21 bp | 3'-host genome |
| Probe | MZHG0JG probe / | FAM-TTAAGTTGTCTAAGCGTCAATTTG-BHQ | 24 bp |
