QT-EVE-ZM-026
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 4114 | DP-004114-3 (Maize)
|
| Genetic target: | 4114 insert |
| Description: | Quantitative PCR method for detection of maize event 4114 (EURL GMFF, 2018) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
GCTTTGGAGCCTCTCGTTTGTANCACTTGCACGTAGTTACCCGGACCGAANNTTCAACACAGATCTGATAGTTTAAACGC |
| Amplicon size: | 80 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | PHN164689 / | GCTTTGGAGCCTCTCGTTTGTA | 22 bp | 5'-host genome |
| Primer reverse | PHN1641690 / | GCGTTTAAACTATCAGATCTGTGTTGAA | 28 bp | insert |
| Probe | PHN164691 / | FAM-CACTTGCACGTAGTTACCCGGACCGAA-BHQ | 27 bp |
