QT-EVE-ZM-025
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON87403 | MON-87403-1 (Maize)
|
| Genetic target: | MON87403 insert |
| Description: | Quantitative PCR method for detection of maize event MON87403 (EURL GMFF, 2018) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CTTTCTTTTTCTCCATATTGACCATCATACTCATTGCGATCCACATTTCCCTACATGGNNNNNGATACAGAGCACTCATT CCGGAGTA |
| Amplicon size: | 88 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 87403 QF / | CTTTCTTTTTCTCCATATTGACCATCATAC | 30 bp | insert |
| Primer reverse | 87403 QR / | TACTCCGGAATGAGTGCTCTGTATC | 25 bp | 3'-host genome |
| Probe | 87403 QP / | FAM-TCATTGCGATCCACATTTCCCTACATGG-TAMRA | 28 bp |
