QT-EVE-ZM-024
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON87411 | MON-87411-9 (Maize)
|
| Genetic target: | MON87411 insert |
| Description: | Quantitative PCR method for detection of maize event MON87411 (EURL GMFF, 2016) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CTCTGTAACAGAAAACACCATCTAGAGNNNCCGCGTTTAAACTATCAGTGTTTAGAGAATNNNNNNNNNNNNNNNNNNNN NATCTACCCTAGAACTAGTTCACTTTTGT |
| Amplicon size: | 109 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 87411 QF / | CTCTGTAACAGAAAACACCATCTAGAG | 27 bp | insert |
| Primer reverse | 87411 QR / | ACAAAAGTGAACTAGTTCTAGGGTAGAT | 28 bp | 3'-host genome |
| Probe | 87411 QP / | FAM-CCGCGTTTAAACTATCAGTGTTTAGAGAAT-TAMRA | 30 bp |
