QT-EVE-ZM-023
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt176 (176) | SYN-EV176-9 (Maize)
|
| Genetic target: | Bt176 insert |
| Description: | Quantitative PCR method for detection of maize event Bt176 (EURL GMFF, 2011) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GGCCGTGAACGAGCTGTTNNNNNNNAGCAACCAGATCGGCCGACACCNNNNNNNNNNTTAGAAAACATGTAGGCTTCTTC CC |
| Amplicon size: | 82 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Bt176 F / | GGCCGTGAACGAGCTGTT | 18 bp | insert |
| Primer reverse | Bt176 R / | GGGAAGAAGCCTACATGTTTTCTAA | 25 bp | 3'-host genome |
| Probe | Bt-176 Probe / | FAM-AGCAACCAGATCGGCCGACACC-TAMRA | 22 bp |
