QT-EVE-ZM-021
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 98140 | DP-098140-6 (Maize)
|
| Genetic target: | 98140 insert |
| Description: | Quantitative PCR method for detection of maize event 98140 (EURL GMFF, 2011) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GTGTGTATGTCTCTTTGCTTGGTCTTNCTCTATCGATCCCCCTCTTTGATAGTTTAAACTGAAGGCGGGAAACGACAATC |
| Amplicon size: | 80 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | DP098-f6 / | GTGTGTATGTCTCTTTGCTTGGTCTT | 26 bp | 5'-host genome |
| Primer reverse | DP098-r2 / | GATTGTCGTTTCCCGCCTTC | 20 bp | insert |
| Probe | DP098-p5 / | FAM-CTCTATCGATCCCCCTCTTTGATAGTTTAAACT-TAMRA | 33 bp |
