QT-EVE-ZM-020
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON810 | MON-00810-6 (Maize)
|
| Genetic target: | MON810 insert |
| Description: | Quantitative PCR method for detection of maize event MON810 (EURL GMFF, 2009) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TCGAAGGACGAAGGACTCTAACGTTTAACATCCTTTGCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAA AAGGAAGGTGGC Genbank ID: AF434709 |
| Amplicon size: | 92 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Mail-F1 / | TCGAAGGACGAAGGACTCTAACGT | 24 bp | 5'-host genome |
| Primer reverse | Mail-R1 / | GCCACCTTCCTTTTCCACTATCTT | 24 bp | insert |
| Probe | Probe Mail-S2 / | FAM-AACATCCTTTGCCATTGCCCAGC-TAMRA | 23 bp |
