QT-EVE-ZM-019
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 3272 | SYN-E3272-5 (Maize)
|
| Genetic target: | 3272 insert |
| Description: | Quantitative PCR method for detection of maize event 3272 (EU-RL GMFF, 2008) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TCATCAGACCAGATTCTCTTTTATGGNNNNNNNNNNNNNNNNNNNNACTGCTGACGCGGCCAAACACTGNNNNNNTAAAC TGAAGGCGGGAAACG |
| Amplicon size: | 95 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | ES3272-F / | TCATCAGACCAGATTCTCTTTTATGG | 26 bp | 5'-host genome |
| Primer reverse | ES3272-R / | CGTTTCCCGCCTTCAGTTTA | 20 bp | insert |
| Probe | ES3272-P / | FAM-ACTGCTGACGCGGCCAAACACTG-TAMRA | 23 bp |
