QT-EVE-ZM-018
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON89034 | MON-89034-3 (Maize)
|
| Genetic target: | MON89034 insert |
| Description: | Quantitative PCR method for detection of maize event MON89034 (EURL GMFF, 2008) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TTCTCCATATTGACCATCATACTCATTNNATCCCCGGAAATTATGTTNNTTTAAAAACCACGGTATTATAGATACCG |
| Amplicon size: | 77 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MON 89034 primer 1 / | TTCTCCATATTGACCATCATACTCATT | 27 bp | insert |
| Primer reverse | MON 89034 primer 2 / | CGGTATCTATAATACCGTGGTTTTTAAA | 28 bp | 3'-host genome |
| Probe | MON 89034 probe / | FAM-ATCCCCGGAAATTATGTT-MGBNFQ | 18 bp |
