QT-EVE-ZM-017
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event LY038 | REN-00038-3 (Maize)
|
| Genetic target: | LYO38 insert |
| Description: | Quantitative PCR method for detection of maize event LY038 (EURL GMFF, 2008) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TGGGTTCAGTCTGCGAATGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAGCGGAGTTTATGGGTCG ACGGNNTCGATAAGCTTGATATCGAATTCCT |
| Amplicon size: | 111 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | LY038 AF / | TGGGTTCAGTCTGCGAATGTT | 21 bp | 5'-host genome |
| Primer reverse | LY038 AR / | AGGAATTCGATATCAAGCTTATCGA | 25 bp | insert |
| Probe | LY038 AP / | FAM-CGAGCGGAGTTTATGGGTCGACGG-TAMRA | 24 bp |
