QT-EVE-ZM-016
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON88017 | MON-88017-3 (Maize)
|
| Genetic target: | MON88017 insert |
| Description: | Quantitative PCR method for detection of maize event MON88017 (EURL GMFF, 2013) |
| Comments: | There is a mismatch between the sequence T of the reverse primer and the reported genomic sequence C of MON88017 (postion 94 in the amplicon sequence). According to Monsanto this does not have a bad influence on the PCR and the method has been validated. |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GAGCAGGACCTGCAGAAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNTCCCGCCTTCAGTTTAAACAGAGTCGGGTNTGGA TGGTCAACTCCGGC |
| Amplicon size: | 94 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MON 88017 AF / | GAGCAGGACCTGCAGAAGCT | 20 bp | insert |
| Primer reverse | MON 88017 AR / | TCCGGAGTTGACCATCCA | 18 bp | 3'-host genome |
| Probe | MON 88017 AP / | FAM-TCCCGCCTTCAGTTTAAACAGAGTCGGGT-TAMRA | 29 bp |
