QT-EVE-ZM-015
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt11 | SYN-BT011-1 (Maize)
|
| Genetic target: | Bt11 insert |
| Description: | Quantitative PCR method for detection of maize event Bt11 (EURL GMFF, 2008) |
| Comments: | This method was also verified in the context of the maize GMO stack Bt11 x MIR162 x TC1507 x NK603 (EURL-VL-02/24) in a bridging study, applying the relative quantification with a two-standard curve approach instead of the originally validated ∆Cq approach and using Rox as passive reference instead of sulforhodamine. |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TGTGTGGCCATTTATCATCGANTTCCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCG |
| Amplicon size: | 68 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Bt11-ev-f1 / | TGTGTGGCCATTTATCATCGA | 21 bp | 5'-host genome |
| Primer reverse | Bt11-ev-r5 / | CGCTCAGTGGAACGAAAACTC | 21 bp | insert |
| Probe | Bt11-ev-p1 / | FAM-TTCCATGACCAAAATCCCTTAACGTGAGT-TAMRA | 29 bp |
