QT-EVE-ZM-013
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MIR604 | SYN-IR604-5 (Maize)
|
| Genetic target: | MIR604 insert |
| Description: | Quantitative PCR method for detection of maize event MIR604 (EURL GMFF, 2013) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GCGCACGCAATTCAACAGNAGGCGGGAAACGACAATCTGATCATGNNNNNAGAATTAAGGGAGTCACGTTATGACC |
| Amplicon size: | 76 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MIR604 primer F / | GCGCACGCAATTCAACAG | 18 bp | 5'-host genome |
| Primer reverse | MIR604 primer R / | GGTCATAACGTGACTCCCTTAATTCT | 26 bp | insert |
| Probe | MIR604 probe / | FAM-AGGCGGGAAACGACAATCTGATCATG-TAMRA | 26 bp |
