QT-EVE-ZM-009
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON863 | MON-00863-5 (Maize)
|
| Genetic target: | MON863 insert |
| Description: | Quantitative PCR method for detection of maize event MON863 (EURL GMFF, 2005) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TGTTACGGCCTAAATGCTGAACTNNTGACCCTACTTGTTCGGATGGGTGTTCANNNNNNNNNGTACCAAGCTTTCCGATC CTAC |
| Amplicon size: | 84 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MON 863 primer F / | TGTTACGGCCTAAATGCTGAACT | 23 bp | 5'-host genome |
| Primer reverse | MON 863 primer R / | GTAGGATCGGAAAGCTTGGTAC | 22 bp | insert |
| Probe | MON 863 probe / | FAM-TGAACACCCATCCGAACAAGTAGGGTCA-TAMRA | 28 bp |
