QT-EVE-ZM-008
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event NK603 | MON-00603-6 (Maize)
|
| Genetic target: | NK603 insert |
| Description: | Quantitative PCR method for detection of maize event NK603 (EURL GMFF, 2005) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
ATGAATGACCTCGAGTAAGCTTGTTAANNNNNNNNNNNNNNNNNNNNNNNNNNTGGTACCACGCGACACACTTCCACTCN AGTGTTTGAGTGGATCCTGTTATCTCTT |
| Amplicon size: | 108 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | NK603-F / | ATGAATGACCTCGAGTAAGCTTGTTAA | 27 bp | insert |
| Primer reverse | NK603-R / | AAGAGATAACAGGATCCACTCAAACACT | 28 bp | 3'-host genome |
| Probe | NK603-PR / | FAM-TGGTACCACGCGACACACTTCCACTC-TAMRA | 26 bp |
