QT-EVE-ZM-007
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event GA21 (G21) | MON-00021-9 (Maize)
|
| Genetic target: | GA21 insert |
| Description: | Quantitative PCR method for detection of maize event GA21 (EURL GMFF, 2005) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CTTATCGTTATGCTATTTGCAACTTTAGANCATATACTAACTCATATCTCTTTCTCAACAGCAGGTGGGTNNNNNNNNNN NNNNNNNNNNNNNNNAGGAGGATCGCGAGCCA |
| Amplicon size: | 112 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | GA21 primer F / | CTTATCGTTATGCTATTTGCAACTTTAGA | 29 bp | 5'-host genome |
| Primer reverse | GA21 primer R / | TGGCTCGCGATCCTCCT | 17 bp | insert |
| Probe | GA21 probe PR / | FAM-CATATACTAACTCATATCTCTTTCTCAACAGCAGGTGGGT-TAMRA | 40 bp |
