QT-EVE-ZM-002
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 5307 | SYN-05307-1 (Maize)
|
| Genetic target: | 5307 insert |
| Description: | Quantitative PCR method for detection of maize event 5307 (EU-RL GMFF, 2014) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CATGGCCGTATCCGCAATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCACAATATACCCTCTTCCCTGGGCC AGNNNNNNNCCACTGGCAAAGGGTGCA |
| Amplicon size: | 107 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 5307i3' forward primer / | CATGGCCGTATCCGCAATGTG | 21 bp | insert |
| Primer reverse | 5307i3' reverse primer / | TGCACCCTTTGCCAGTGG | 18 bp | 3'-host genome |
| Probe | 5307i3'-s2 probe / | FAM-ACCACAATATACCCTCTTCCCTGGGCCAG-TAMRA | 29 bp |
