QT-EVE-ZM-001
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event VCO-01981-5 | VCO-01981-5 (Maize)
|
| Genetic target: | VCO-01981-5 insert |
| Description: | Quantitative PCR method for detection of maize event VCO-01981-5 (EURL GMFF, 2016) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CCACTGAACGTCACCAAGAAGANCAGTACTCAAACACTGATAGNNNNNNNNNNNNNNNNNNNNNNTAAATCCCTCGAGTA GCGGC |
| Amplicon size: | 85 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | VCO-01981-5 primer F / | CCACTGAACGTCACCAAGAAGA | 22 bp | 5'-host genome |
| Primer reverse | VCO-01981-5 primer R / | GCCGCTACTCGAGGGATTTA | 20 bp | insert |
| Probe | VCO-01981-5 probe / | FAM-CAGTACTCAAACACTGATAG-MGB | 20 bp |
