QT-EVE-GM-021
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event COR23134 | COR-23134-4 (Soybean)
|
| Genetic target: | 5' host genome / COR23134 insert |
| Description: | Quantitative PCR method for detection of soybean event COR23134 |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GACTTGCGTTAGTTGTGTTAGAATGTCNNNCGGATAGTTATATTTGTGCCAACNNNNAAACATCAAAAAAATCGGGATCA |
| Amplicon size: | 80 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | COR-23134-4 Forward Primer / | GACTTGCGTTAGTTGTGTTAGAATGTC | 27 bp | 5'-host genome |
| Primer reverse | COR-23134-4 Reverse Primer / | TGATCCCGATTTTTTTGATGTTT | 23 bp | insert |
| Probe | COR-23134-4 Probe / | 6-FAM-CGGATAGTTATATTTGTGCCAAC-MGBNFQ | 23 bp |
